Application
Immunohistochemistry staining of Caspase-7 in skeletal muscle tissue using Caspase-7 monoclonal Antibody.| Product Name | Caspase-7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP7, Apoptotic protease Mch-3, Caspase 7, Caspase-7, ICE-LAP3, LICE2, MCH3, ICE-like apoptotic protease 3, CASP-7, CMH-1 |
| Product description | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-7 is derived from the cleavage of proMCH3-alpha into 2 subunits, p20 and p12 by Caspase-3. Sequence analysis revealed that the MCH3-alpha amino acid sequence had the highest homology to caspase-3 (53% identity) and less than 37% identity with all other family members. Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-7 is an executioner caspase that was identified based on its homology with caspases 1 and 3, as well as the C. elegans cell death protein CED-3. Alternative splicing of Caspase-7 mRNA results in the production of 3 distinct isoforms. Caspase-7 activity can be directly inhibited by XIAP expression. |
| Immunogen | Recombinant human Caspase-7. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| Caspase-7 antibody can be used in ELISA, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CASP7 |
|---|---|
| Alternative Names | CASP7 Apoptotic protease Mch-3 Caspase 7 Caspase-7 ICE-LAP3 LICE2 MCH3 ICE-like apoptotic protease 3 CASP-7 CMH-1 |
| Tissue Specificity | In Jurkat, a 35 kD band is observed. |
Application
Immunohistochemistry staining of Caspase-7 in skeletal muscle tissue using Caspase-7 monoclonal Antibody.| Application Notes | Caspase-7 antibody can be used in ELISA, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-7 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS containing 0.2% gelatin and 0.05% sodium azide. |
Data sheet for OM273519