Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1.0 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-8.| Product Name | IL-8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, C-X-C motif chemokine 8, IL-8 |
| Product description | This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-8 (human Interleukin-8). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: To detect hIL-8 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-8. Sandwich: To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8. Western Blot: To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL8 |
|---|---|
| Alternative Names | NAF GCP1 LECT LUCT NAP1 CXCL8 GCP-1 LYNAP MDNCF MONAP NAP-1 Interleukin-8 C-X-C motif chemokine 8 IL-8 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1.0 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-8.| Application Notes | ELISA: To detect hIL-8 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-8. Sandwich: To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-8. Western Blot: To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-8 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM282010