Interleukin-8 (IL-8) is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.1) Baggiolini M. et al. 1991. FEBS Lett. 307:97-101.
Immunogen
Ni-NTA purified recombinant human IL-8 expressed in E.Coli. strain BL21 (DE3).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ELISAWB
Western Blot:1:500 - 1,000 ELISA:1,000 - 2,000. Determining optimal working dilutions by titration test.:
Western blot analysis using IL - 8 monoclonal antibody against truncated IL - 8 recombinant protein.
Application Notes
Western Blot:1:500 - 1,000 ELISA:1,000 - 2,000. Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
IL-8 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Purified antibody in PBS containing 0.03% sodium azide