| Product Name | IL-8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin 8, CXCL8, Interleukin-8, C-X-C motif chemokine 8, IL-8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1 |
| Product description | IL-8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes. |
| Immunogen | The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-8 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-8 when used with our biotinylated antigen affinity purified anti-human IL-8 (XP-5199Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL8 |
|---|---|
| Alternative Names | Interleukin 8 CXCL8 Interleukin-8 C-X-C motif chemokine 8 IL-8 NAF GCP1 LECT LUCT NAP1 GCP-1 LYNAP MDNCF MONAP NAP-1 |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-8 when used with our biotinylated antigen affinity purified anti-human IL-8 (XP-5199Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | IL-8 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM282013