WB
Western blot analysis of extracts of various cell lines, using COMP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IF-P
Immunofluorescence analysis of Rat cartilage using COMP Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.| Product Name | COMP Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COMP |
|---|---|
| Gene Synonyms | MED CTS2 EDM1 EPD1 TSP5 PSACH THBS5 TSP-5 |
| Gene Full Name | cartilage oligomeric matrix protein |
| Gene Summary | The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016] |
| Molecular Weight(MW) | 83kDa(Observed MW 110kDa) |
| Cellular Localization | Secreted,extracellular matrix,extracellular space |
WB
Western blot analysis of extracts of various cell lines, using COMP antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IF-P
Immunofluorescence analysis of Rat cartilage using COMP Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
Data sheet for OM643177