WB
Various lysates were subjected to SDS PAGE followed by western blot with COMP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using COMP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Product Name | COMP Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human COMP protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COMP |
|---|---|
| Gene Synonyms | MED CTS2 EDM1 EPD1 TSP5 PSACH THBS5 TSP-5 |
| Gene Full Name | cartilage oligomeric matrix protein |
| Gene Summary | The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016] |
| Molecular Weight(MW) | 80-110kDa |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with COMP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using COMP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM644030