Application
Lanes: 1. 100 ug mouse testis lysate2. 100 ug HeLa cell lysatePrimary Antibody Dilution: 1:800Secondary Antibody: Anti-rabbit-APSecondary Antibody Dilution: 1:10000Gene Name: IKBKGSubmitted by: Andreia Carvalho, Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication
WB Suggested Anti-IKBKG Antibody Titration: 1.25ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateIKBKG is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells| Product Name | Rabbit anti-IKBKG polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma |
| Immunogen | The immunogen for anti-IKBKG antibody: synthetic peptide directed towards the middle region of human IKBKG |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IKBKG |
|---|---|
| Gene Synonyms | IKBKG |
| Gene Full Name | Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma |
| Gene Summary | Familial incontinentia pigmenti (IP) is a genodermatosis that segregates as an X-linked dominant disorder and is usually lethal prenatally in males . In affected females it causes highly variable abnormalities of the skin, hair, nails, teeth, eyes, and central nervous system. The prominent skin signs occur in 4 classic cutaneous stages: perinatal inflammatory vesicles, verrucous patches, a distinctive pattern of hyperpigmentation, and dermal scarring. Cells expressing the mutated X chromosome are eliminated selectively around the time of birth, so females with IP exhibit extremely skewed X-inactivation. Familial incontinentia pigmenti is caused by mutations in the NEMO gene and is here referred to as IP2, or 'classical' incontinentia pigmenti. Sporadic incontinentia pigmenti, the so-called IP1, which maps to Xp11, is categorized as hypomelanosis of Ito |
| Alternative Names | IP IP1 IP2 FIP3 IPD2 NEMO FIP-3 Fip3p AMCBX1 IKK-gamma |
| Molecular Weight(MW) | 48kDa |
| Sequence | 419 amino acids |
Application
Lanes: 1. 100 ug mouse testis lysate2. 100 ug HeLa cell lysatePrimary Antibody Dilution: 1:800Secondary Antibody: Anti-rabbit-APSecondary Antibody Dilution: 1:10000Gene Name: IKBKGSubmitted by: Andreia Carvalho, Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) Group
Application
WB Suggested Anti-IKBKG Antibody Titration: 1.25ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateIKBKG is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM105096