Immunoblot
Lane1: 30 ug untreated human HCT116 cell lysateLane2-3: 30 ug genotoxic agent treated human HCT116 cell lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CDK4Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University JenaImmunoblot
1. 30 ug human HCT116 cell lysate2. 30 ug genotoxic treated human HCT116 cell lysate3. 30 ug genotoxic treated human HCT116 cell lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CDK4Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Friedrich-schiller University JenaWestern blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Product Name | Rabbit anti-CDK4 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMM3, MGC14458, PSK-J3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanPigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK4 |
|---|---|
| Gene Synonyms | CMM3 MGC14458 PSK-J3 |
| Gene Full Name | Cyclin-dependent kinase 4 |
| Gene Summary | Cdk4 is probably involved in the control of the cell cycle. |
| Alternative Names | CMM3 MGC14458 PSK-J3 |
| Molecular Weight(MW) | 34kDa |
| Sequence | 303 amino acids |
Immunoblot
Lane1: 30 ug untreated human HCT116 cell lysateLane2-3: 30 ug genotoxic agent treated human HCT116 cell lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CDK4Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena
Immunoblot
1. 30 ug human HCT116 cell lysate2. 30 ug genotoxic treated human HCT116 cell lysate3. 30 ug genotoxic treated human HCT116 cell lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CDK4Submitted by: Anke Rauch & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Friedrich-schiller University Jena
Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
