Immunoblot
Lane1: 50 ug HCT116 cell lysateLane2: 50 ug HT-29 cell lysateLane3: 50 ug T47D cell lysateLane4: 50 ug HEK-293T cell lysateLane5: 50 ug MCF7 cell lysateLane6: 50 ug MDA-MB-231 cell lysatePrimary Antibody Dilution: 2 ug/mlSecondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: Gene Name: RELASubmitted by: AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysateWestern blot
RELA antibody - middle region validated by WB using HCT116/Ht-29/T47D/HEK-293T/MCF/MDA-MB-231 cell lines at 2ug/ml.| Product Name | Rabbit anti-RELA polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC131774, NFKB3, p65 |
| Immunogen | The immunogen for anti-RELA antibody: synthetic peptide directed towards the middle region of human RELA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RELA |
|---|---|
| Gene Synonyms | MGC131774 NFKB3 p65 |
| Gene Full Name | V-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| Gene Summary | p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767 |
| Alternative Names | MGC131774 NFKB3 p65 |
| Molecular Weight(MW) | 60kDa |
| Sequence | 551 amino acids |
Immunoblot
Lane1: 50 ug HCT116 cell lysateLane2: 50 ug HT-29 cell lysateLane3: 50 ug T47D cell lysateLane4: 50 ug HEK-293T cell lysateLane5: 50 ug MCF7 cell lysateLane6: 50 ug MDA-MB-231 cell lysatePrimary Antibody Dilution: 2 ug/mlSecondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: Gene Name: RELASubmitted by: Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate
Western blot
RELA antibody - middle region validated by WB using HCT116/Ht-29/T47D/HEK-293T/MCF/MDA-MB-231 cell lines at 2ug/ml.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
