Immunohistochemical
Sample Type: human colon tissues infected ex-vivo with HIV-1Green: PrimaryBlue: DAPIPrimary Dilution: 1:100Secondary Antibody: Donkey anti-Rabbit AF 488Secondary Dilution: 1:500Image Submitted By: Chiara FoglieniSan Raffaele Scientific Institute, Milan, ItalyImmunohistochemistry
Immunohistochemistry with HMEC-1 and A549 cells tissueWestern blot
Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at BirminghamWestern blot
CXCR4 antibody - N-terminal region validated by WB using 721_B cell Lysate at 0.2-1 ug/ml.Western blot
CXCR4 antibody - N-terminal region validated by WB using human microvascular endothelial cells (25ug) at 1:1000.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-CXCR4 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD184, D2S201E, FB22, HM89, HSY3RR, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM |
| Immunogen | The immunogen for anti-CXCR4 antibody: synthetic peptide directed towards the N terminal of human CXCR4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| FC:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCR4 |
|---|---|
| Gene Synonyms | CD184 D2S201E FB22 HM89 HSY3RR LAP3 LCR1 LESTR NPY3R NPYR NPYRL NPYY3R WHIM |
| Gene Full Name | Chemokine (C-X-C motif) receptor 4 |
| Gene Summary | CXCR4 is a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Alternative Names | CD184 D2S201E FB22 HM89 HSY3RR LAP3 LCR1 LESTR NPY3R NPYR NPYRL NPYY3R WHIM |
| Molecular Weight(MW) | 40kDa |
| Sequence | 352 amino acids |
Immunohistochemical
Sample Type: human colon tissues infected ex-vivo with HIV-1Green: PrimaryBlue: DAPIPrimary Dilution: 1:100Secondary Antibody: Donkey anti-Rabbit AF 488Secondary Dilution: 1:500Image Submitted By: Chiara FoglieniSan Raffaele Scientific Institute, Milan, Italy
Immunohistochemistry
Immunohistochemistry with HMEC-1 and A549 cells tissue
Western blot
Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:5,000Submitted by: Scott Wilson, University of Alabama at Birmingham
Western blot
CXCR4 antibody - N-terminal region validated by WB using 721_B cell Lysate at 0.2-1 ug/ml.
Western blot
CXCR4 antibody - N-terminal region validated by WB using human microvascular endothelial cells (25ug) at 1:1000.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Application Notes | FC:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM105220