Immunohistochemistry
Immunohistochemistry with Prostate tissue at an antibody concentration of 5ug/ml using anti-AURKA antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:2500Positive Control: 293T cell lysate| Product Name | Rabbit anti-AURKA polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, PPP1R47 |
| Immunogen | The immunogen for anti-AURKA antibody: synthetic peptide directed towards the C terminal of human AURKA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AURKA |
|---|---|
| Gene Synonyms | AIK ARK1 AURA AURORA2 BTAK MGC34538 STK15 STK6 STK7 PPP1R47 |
| Gene Full Name | Aurora kinase A |
| Gene Summary | AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. It is found at the centrosome in interphase cells and at the spindle poles in mitosis. This protein may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. |
| Alternative Names | AIK ARK1 AURA AURORA2 BTAK MGC34538 STK15 STK6 STK7 PPP1R47 |
| Molecular Weight(MW) | 46kDa |
| Sequence | 403 amino acids |
Immunohistochemistry
Immunohistochemistry with Prostate tissue at an antibody concentration of 5ug/ml using anti-AURKA antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:2500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
