Western blot
0.2-1 ug/mlPositive Control: Transfected 293T| Product Name | Rabbit anti-NFYB polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBF-A, CBF-B, HAP3, NF-YB |
| Immunogen | The immunogen for anti-NFYB antibody: synthetic peptide directed towards the middle region of human NFYB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFYB |
|---|---|
| Gene Synonyms | CBF-A CBF-B HAP3 NF-YB |
| Gene Full Name | Nuclear transcription factor Y, beta |
| Gene Summary | NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. |
| Alternative Names | CBF-A CBF-B HAP3 NF-YB |
| Molecular Weight(MW) | 23kDa |
| Sequence | 207 amino acids |
Western blot
0.2-1 ug/mlPositive Control: Transfected 293T| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
