Immunofluorescence
Mouse retina Primary Antibody Dilution : 1:200 Secondary Antibody :Donkey anti rabbit-Cy3Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions :Red: PPARGC1AGene Name :PPARGC1ASubmitted by :Dr. Joshua SanesWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-PPARGC1A polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LEM6, PGC-1(alpha), PGC-1v, PGC1, PGC1A, PPARGC1 |
| Immunogen | The immunogen for anti-PPARGC1A antibody: synthetic peptide directed towards the N terminal of human PPARGC1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGoatHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPARGC1A |
|---|---|
| Gene Synonyms | LEM6 PGC-1(alpha) PGC-1v PGC1 PGC1A PPARGC1 |
| Gene Full Name | Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
| Gene Summary | PPARGC1A is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | LEM6 PGC-1(alpha) PGC-1v PGC1 PGC1A PPARGC1 |
| Molecular Weight(MW) | 91kDa |
| Sequence | 798 amino acids |
Immunofluorescence
Mouse retina Primary Antibody Dilution : 1:200 Secondary Antibody :Donkey anti rabbit-Cy3Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions :Red: PPARGC1AGene Name :PPARGC1ASubmitted by :Dr. Joshua Sanes
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Application Notes | IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
