Immunoblot
Lane 1: 25 ug empty vector transfected H460 cell lysateLane 2: 25 ug hGATA5 transfected H460 cell lysatePrimary Antibody Dilution: 1:1200Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3500Gene Name: GATA5Submitted by: AnonymousWestern blot
0.2-1 ug/mlPositive Control: HT1080 cell lysate| Product Name | Rabbit anti-GATA5 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bB379O24.1 |
| Immunogen | The immunogen for anti-GATA5 antibody: synthetic peptide directed towards the middle region of human GATA5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA5 |
|---|---|
| Gene Synonyms | bB379O24.1 |
| Gene Full Name | GATA binding protein 5 |
| Gene Summary | The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. |
| Alternative Names | bB379O24.1 |
| Molecular Weight(MW) | 41kDa |
| Sequence | 397 amino acids |
Immunoblot
Lane 1: 25 ug empty vector transfected H460 cell lysateLane 2: 25 ug hGATA5 transfected H460 cell lysatePrimary Antibody Dilution: 1:1200Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3500Gene Name: GATA5Submitted by: Anonymous
Western blot
0.2-1 ug/mlPositive Control: HT1080 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
