Immunohistochemical
ImmunohistochemicalWestern blot
Lane 1: 20ug U87 lysate Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:2000 Color/Signal Descriptions:SOX2 Gene Name:Ander Matheu Fernandez, Biodonostia Institute Submitted by:Western blot
0.2-1 ug/mlELISA Titer: 1:500Positive Control: OVCAR-3 cell lysate| Product Name | Rabbit anti-SOX2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANOP3, MCOPS3, MGC2413 |
| Immunogen | The immunogen for anti-SOX2 antibody: synthetic peptide directed towards the N terminal of human SOX2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOX2 |
|---|---|
| Gene Synonyms | ANOP3 MCOPS3 MGC2413 |
| Gene Full Name | SRY (sex determining region Y)-box 2 |
| Gene Summary | SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | ANOP3 MCOPS3 MGC2413 |
| Molecular Weight(MW) | 34kDa |
| Sequence | 317 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
Lane 1: 20ug U87 lysate Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:2000 Color/Signal Descriptions:SOX2 Gene Name:Ander Matheu Fernandez, Biodonostia Institute Submitted by:
Western blot
0.2-1 ug/mlELISA Titer: 1:500Positive Control: OVCAR-3 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
