Immunoblot
Lane 1: 5ug of transfected 293T lysate (SMAD1)
Lane 1: 5ug of transfected 293T lysate (SMAD2)
Lane 1: 5ug of transfected 293T lysate (SMAD3)
Lane 1: 5ug of transfected 293T lysate (SMAD4)
Lane 1: 5ug of transfected 293T lysate (SMAD5)
Lane 1: 5ug of transfected 293T lysate (SMAD6)
Lane 1: 5ug of transfected 293T lysate (SMAD7)
Lane 1: 5ug of transfected 293T lysate (SMAD8)
Lane 1: 5ug of transfected 293T lysate (GFP)
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit IgG HRP Conjugated
Secondary Antibody Dilution:
1:10,000
Gene Name:
SMAD5
Submitted by:
Amanda Urick, Medical College of Wisconsin
Western blot
0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate