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SMAD5 antibody - middle region

Catalog Number:OM105594
OM105594
Discount Price
OM105594-100ul
1~2 Week
100ul
OM105594-50ul
50ul
OM105594-20ul
20ul
1~2 Week
Product Profile
Product Name SMAD5 antibody - middle region
Antibody Type Primary Antibodies
Immunogen The immunogen for anti-SMAD5 antibody: synthetic peptide directed towards the middle region of human SMAD5
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications WB
Species Reactivity Bovine,Dog,Horse,Human,Mouse,Rat,Zebra Fish
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol SMAD5
Gene Synonyms DWFC
JV5-1
MADH5
Gene Full Name SMAD family member 5
Gene Summary SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
Alternative Names DWFC
JV5-1
MADH5
Molecular Weight(MW) 51kDa
Sequence 465 amino acids
Database Links
Entrez Gene 4090
SwissProt ID Q99717
Protein Accession NP_005894
Application

Immunoblot

Lane 1: 5ug of transfected 293T lysate (SMAD1)
Lane 1: 5ug of transfected 293T lysate (SMAD2)
Lane 1: 5ug of transfected 293T lysate (SMAD3)
Lane 1: 5ug of transfected 293T lysate (SMAD4)
Lane 1: 5ug of transfected 293T lysate (SMAD5)
Lane 1: 5ug of transfected 293T lysate (SMAD6)
Lane 1: 5ug of transfected 293T lysate (SMAD7)
Lane 1: 5ug of transfected 293T lysate (SMAD8)
Lane 1: 5ug of transfected 293T lysate (GFP)
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit IgG HRP Conjugated
Secondary Antibody Dilution:
1:10,000
Gene Name:
SMAD5
Submitted by:
Amanda Urick, Medical College of Wisconsin

Western blot

0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
Additional Information
Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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