Western blot
Positive Control: Lane 1: 2ug Flag-SMAD7 transfected 293 extracts Primary Antibody Dilution : 1:500Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Anonymous| Product Name | Rabbit anti-SMAD7 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRCS3, MADH7, MADH8 |
| Immunogen | The immunogen for anti-SMAD7 antibody: synthetic peptide directed towards the N terminal of human SMAD7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD7 |
|---|---|
| Gene Synonyms | CRCS3 MADH7 MADH8 |
| Gene Full Name | SMAD family member 7 |
| Gene Summary | Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition. |
| Alternative Names | CRCS3 MADH7 MADH8 |
| Molecular Weight(MW) | 46kDa |
| Sequence | 426 amino acids |
Western blot
Positive Control: Lane 1: 2ug Flag-SMAD7 transfected 293 extracts Primary Antibody Dilution : 1:500Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Anonymous| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
