Western blot
Host: Rabbit Target Name:GLI1 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:5.0ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 6%-18%Western blot
2.5ug/mlPositive Control: HepG2 cell lysateWestern blot
1.0ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-GLI1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLI |
| Immunogen | The immunogen for anti-GLI1 antibody: synthetic peptide directed towards the C terminal of human GLI1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GLI1 |
|---|---|
| Gene Synonyms | GLI |
| Gene Full Name | GLI family zinc finger 1 |
| Gene Summary | GLI1 is a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined; however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation. |
| Alternative Names | GLI |
| Molecular Weight(MW) | 118kDa |
| Sequence | 1106 amino acids |
Western blot
Host: Rabbit Target Name:GLI1 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:5.0ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 6%-18%
Western blot
2.5ug/mlPositive Control: HepG2 cell lysate
Western blot
1.0ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
