Immunoprecipitation
K562Western blot
HES1 antibody - N-terminal region validated by WB using Mouse embryo tissue at 1:800.Western blot
HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.Western blot
1.25ug/mlPositive Control: Raji cell lysate| Product Name | Rabbit anti-HES1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HHL, HRY, HES-1, bHLHb39 |
| Immunogen | The immunogen for anti-HES1 antibody: synthetic peptide directed towards the N terminal of human HES1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:500~1:2000 IHC:1:50~1:200 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HES1 |
|---|---|
| Gene Synonyms | HHL HRY HES-1 bHLHb39 |
| Gene Full Name | Hairy and enhancer of split 1, (Drosophila) |
| Gene Summary | HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. |
| Alternative Names | HHL HRY HES-1 bHLHb39 |
| Molecular Weight(MW) | 30kDa |
| Sequence | 280 amino acids |
Immunoprecipitation
K562
Western blot
HES1 antibody - N-terminal region validated by WB using Mouse embryo tissue at 1:800.
Western blot
HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.
Western blot
1.25ug/mlPositive Control: Raji cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IF:1:200~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
