Immunoblot
1. 30 ug mouse pancreas extract 2. 30 ug mouse pancreas extract 3. 30 ug mouse pancreas extractPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: SIRT2Submitted by: Dr. Jin Hee Lee, Ph.D., Winthrop-University HospitalWB
(B) SIRT2 protein abundance in OO, CC, GCs and T. The protein expression of SIRT2 was examined by a Western blot analysis. Band intensities normalized to GAPDH are shown.WB
(C) Western blot for SIRT2 is shown after transfection with the piLenti-siRNA-GFP plasmids for 48 h.IHC
(A) SIRT2 localization in a bovine ovarian sample identified via immunochemistry.| Product Name | Rabbit anti-SIRT2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | The immunogen for anti-SIRT2 antibody: synthetic peptide directed towards the C terminal of human SIRT2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT2 |
|---|---|
| Gene Synonyms | SIR2 SIR2L SIR2L2 |
| Gene Full Name | Sirtuin 2 |
| Gene Summary | SIRT2 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. SIRT2 is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene. |
| Alternative Names | SIR2 SIR2L SIR2L2 |
| Molecular Weight(MW) | 40kDa |
| Sequence | 352 amino acids |
Immunoblot
1. 30 ug mouse pancreas extract 2. 30 ug mouse pancreas extract 3. 30 ug mouse pancreas extractPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: SIRT2Submitted by: Dr. Jin Hee Lee, Ph.D., Winthrop-University Hospital
WB
(B) SIRT2 protein abundance in OO, CC, GCs and T. The protein expression of SIRT2 was examined by a Western blot analysis. Band intensities normalized to GAPDH are shown.
WB
(C) Western blot for SIRT2 is shown after transfection with the piLenti-siRNA-GFP plasmids for 48 h.
IHC
(A) SIRT2 localization in a bovine ovarian sample identified via immunochemistry.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
