Immunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: SIRT1Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NEWestern blot
Titration: 0.5 ug/mlPositive Control: Fetal thymus| Product Name | Rabbit anti-SIRT1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIR2L1 |
| Immunogen | The immunogen for anti-SIRT1 antibody: synthetic peptide directed towards the N terminal of human SIRT1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIRT1 |
|---|---|
| Gene Synonyms | SIR2L1 |
| Gene Full Name | Sirtuin 1 |
| Gene Summary | SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; |
| Alternative Names | SIR2L1 |
| Molecular Weight(MW) | 82kDa |
| Sequence | 747 amino acids |
Immunoblot
1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: SIRT1Submitted by: Dr. Pankaj Singh, UNMC, Omaha, NE
Western blot
Titration: 0.5 ug/mlPositive Control: Fetal thymus| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
