Immunoblot
Lane1: 20ug RH30 lysateLane2: 20ug RH30 lysateLane3: 20ug RH41 lysateLane4: 20ug RH41 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-rabbit HRPSecondary Antibody Dilution: 1:4000Gene Name: PAX3Submitted by: Dr Joanna Selfe, Institute of Cancer Research| Product Name | Rabbit anti-PAX3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDHS, HUP2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, WS1, WS3 |
| Immunogen | The immunogen for anti-PAX3 antibody: synthetic peptide directed towards the N terminal of human PAX3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX3 |
|---|---|
| Gene Synonyms | CDHS HUP2 MGC120381 MGC120382 MGC120383 MGC120384 MGC134778 WS1 WS3 |
| Gene Full Name | Paired box 3 |
| Gene Summary | PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. |
| Alternative Names | CDHS HUP2 MGC120381 MGC120382 MGC120383 MGC120384 MGC134778 WS1 WS3 |
| Molecular Weight(MW) | 53kDa |
| Sequence | 484 amino acids |
Immunoblot
Lane1: 20ug RH30 lysateLane2: 20ug RH30 lysateLane3: 20ug RH41 lysateLane4: 20ug RH41 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-rabbit HRPSecondary Antibody Dilution: 1:4000Gene Name: PAX3Submitted by: Dr Joanna Selfe, Institute of Cancer Research| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
