Immunohistochemistry
Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HMGN1 antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-HMGN1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425 |
| Immunogen | The immunogen for anti-HMGN1 antibody: synthetic peptide directed towards the middle region of human HMGN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGN1 |
|---|---|
| Gene Synonyms | FLJ27265 FLJ31471 HMG14 MGC104230 MGC117425 |
| Gene Full Name | High mobility group nucleosome binding domain 1 |
| Gene Summary | Chromosomal protein HMG14 (HMGN1) and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | FLJ27265 FLJ31471 HMG14 MGC104230 MGC117425 |
| Molecular Weight(MW) | 11kDa |
| Sequence | 100 amino acids |
Immunohistochemistry
Immunohistochemistry with Human Liver cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-HMGN1 antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
