Western blot
Sample Type: Primary vascular endothelial cellsProtocol: Loaded 30ug of cell lysate of each. One normal, one cultured with high glucose (which enhances IKK Beta). Expected band is cut. 10% Gel concentration.Primary dilution: 1:1000Western blot
Researcher:Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication:Western blottingSpecies+tissue/cell type:Lane 1: 100ug mouse liver lysateLane 2: 100ug mouse brain lysateLane 3: 100ug mouse heart lysateLane 4: 100ug mouse kidney lysateLane 5: 100ug mouse lung lysateLane 6: 100ug mouse thymus lysateLane 7: 100ug mouse spleen lysateLane 8: 100ug mouse testis lysateLane 9: 100ug HeLa cell lysatePrimary antibody dilution:1:1000Secondary antibody:Anti-rabbit-APSecondary antibody dilution:1:10,000Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain| Product Name | Rabbit anti-IKBKB polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ40509, IKK-beta, IKK2, IKKB, MGC131801, NFKBIKB |
| Immunogen | The immunogen for anti-IKBKB antibody: synthetic peptide directed towards the N terminal of human IKBKB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IKBKB |
|---|---|
| Gene Synonyms | FLJ40509 IKK-beta IKK2 IKKB MGC131801 NFKBIKB |
| Gene Full Name | Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta |
| Gene Summary | NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-203 AL708460.1 9-211 204-3077 AF080158.1 170-3043 3078-3916 AK023193.1 1980-2818 |
| Alternative Names | FLJ40509 IKK-beta IKK2 IKKB MGC131801 NFKBIKB |
| Molecular Weight(MW) | 86kDa |
| Sequence | 756 amino acids |
Western blot
Sample Type: Primary vascular endothelial cellsProtocol: Loaded 30ug of cell lysate of each. One normal, one cultured with high glucose (which enhances IKK Beta). Expected band is cut. 10% Gel concentration.Primary dilution: 1:1000
Western blot
Researcher:Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication:Western blottingSpecies+tissue/cell type:Lane 1: 100ug mouse liver lysateLane 2: 100ug mouse brain lysateLane 3: 100ug mouse heart lysateLane 4: 100ug mouse kidney lysateLane 5: 100ug mouse lung lysateLane 6: 100ug mouse thymus lysateLane 7: 100ug mouse spleen lysateLane 8: 100ug mouse testis lysateLane 9: 100ug HeLa cell lysatePrimary antibody dilution:1:1000Secondary antibody:Anti-rabbit-APSecondary antibody dilution:1:10,000
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
