Western blot
Host: Rabbit Target Name: KLHL12 Sample Tissue: Stomach Tumor lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-KLHL12 Antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C3IP1, DKIR, hDKIR |
| Immunogen | The immunogen for Anti-KLHL12 antibody is: synthetic peptide directed towards the N-terminal region of Human KLHL12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KLHL12 |
|---|---|
| Gene Synonyms | C3IP1 DKIR hDKIR |
| Gene Full Name | kelch-like 12 (Drosophila) |
| Gene Summary | KLHL12 is a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. |
| Alternative Names | C3IP1 DKIR hDKIR |
| Molecular Weight(MW) | 63kDa |
| Sequence | 568 amino acids |
Western blot
Host: Rabbit Target Name: KLHL12 Sample Tissue: Stomach Tumor lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
