Immunoblot
1: 60ug mouse melanocytes (melba), 2: 60ug mouse melanoma (B16), 3: 60ug human melanocytes (HFSC), 4: 60ug human melanoma (SK-MEL5), 5: 60ug human melanoma (YUMAC), 6: 60ug rat shwann cellPrimary Antibody Dilution: 1:200Secondary Antibody: Donkey anti-rabbit HPRTSecondary Antibody Dilution: 1:2000Gene Name: BRD9Submitted by: Dr. Ivana de la Serna, University of ToledoWestern blot
2.5ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-BRD9 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRO9856, LAVS3040 |
| Immunogen | The immunogen for anti-BRD9 antibody: synthetic peptide directed towards the N terminal of human BRD9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BRD9 |
|---|---|
| Gene Synonyms | PRO9856 LAVS3040 |
| Gene Full Name | Bromodomain containing 9 |
| Gene Summary | BRD9 is a member of protein family that contains bromodomain. It is potentially related to cancer. |
| Alternative Names | PRO9856 LAVS3040 |
| Molecular Weight(MW) | 53kDa |
| Sequence | 481 amino acids |
| Entrez Gene | 65980 |
|---|---|
| SwissProt ID | Q9H8M2 |
| Protein Accession | NP_001009877 |
Immunoblot
1: 60ug mouse melanocytes (melba), 2: 60ug mouse melanoma (B16), 3: 60ug human melanocytes (HFSC), 4: 60ug human melanoma (SK-MEL5), 5: 60ug human melanoma (YUMAC), 6: 60ug rat shwann cellPrimary Antibody Dilution: 1:200Secondary Antibody: Donkey anti-rabbit HPRTSecondary Antibody Dilution: 1:2000Gene Name: BRD9Submitted by: Dr. Ivana de la Serna, University of Toledo
Western blot
2.5ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
