Immunoblot
Lane 1: 20ug HEK-293 cell lysateLane 2: 20ug mkchIP2-YFP transfected HEK-293 lysateLane 3: 20ug mkchIP3-YFP transfected HEK-293 lysateLane 4: 20ug mkchIP4-YFP transfected HEK-293 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: KCNIP2Submitted by: Jeanne M. Nerbonne, Arvin Soepriatna, Washington University Medical School Department of Developmental BiologyWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-KCNIP2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp566L1246, KCHIP2, MGC17241 |
| Immunogen | The immunogen for anti-KCNIP2 antibody: synthetic peptide directed towards the N terminal of human KCNIP2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNIP2 |
|---|---|
| Gene Synonyms | DKFZp566L1246 KCHIP2 MGC17241 |
| Gene Full Name | Kv channel interacting protein 2 |
| Gene Summary | KCNIP2 encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belongs to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belongs to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified from this gene. |
| Alternative Names | DKFZp566L1246 KCHIP2 MGC17241 |
| Molecular Weight(MW) | 32kDa |
| Sequence | 285 amino acids |
Immunoblot
Lane 1: 20ug HEK-293 cell lysateLane 2: 20ug mkchIP2-YFP transfected HEK-293 lysateLane 3: 20ug mkchIP3-YFP transfected HEK-293 lysateLane 4: 20ug mkchIP4-YFP transfected HEK-293 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: KCNIP2Submitted by: Jeanne M. Nerbonne, Arvin Soepriatna, Washington University Medical School Department of Developmental Biology
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
