Western blot
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: AnonymousWestern blot
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: Anonymous| Product Name | Rabbit anti-TARDBP polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TDP-43, ALS10 |
| Immunogen | The immunogen for anti-TARDBP antibody: synthetic peptide directed towards the N terminal of human TARDBP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TARDBP |
|---|---|
| Gene Synonyms | TDP-43 ALS10 |
| Gene Full Name | TAR DNA binding protein |
| Gene Summary | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | TDP-43 ALS10 |
| Molecular Weight(MW) | 45kDa |
| Sequence | 414 amino acids |
Western blot
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: Anonymous
Western blot
Positive Control: Lane 1: 5ug mouse brain cytoplasm Lane 2: 5ug mouse brain nucleus Primary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: Anonymous| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
