Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293TWB
(A) Immunoblots for SHP in macrophages after LPS stimulation.WB
(A-C) Fluorescence analysis, immunoblots and qRT-PCR assays for SHP in macrophages infected by croaker SHP adenovirus.WB
(B) Immunoblots for phosphorylated AMPK and SHP in macrophages after LPS stimulation alone or with AMPK inhibitor (CC)WB
(D) Effects of AMPK activator (AICAR) on protein levels of phosphorylated AMPK, phosphorylated ACC and SHP in macrophages.| Product Name | Rabbit anti-NR0B2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-NR0B2 antibody: synthetic peptide directed towards the C terminal of human NR0B2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR0B2 |
|---|---|
| Gene Synonyms | SHP SHP1 |
| Gene Full Name | Nuclear receptor subfamily 0, group B, member 2 |
| Gene Summary | NR0B2 is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. It is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | SHP SHP1 |
| Molecular Weight(MW) | 28kDa |
| Sequence | 257 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Transfected 293T
WB
(A) Immunoblots for SHP in macrophages after LPS stimulation.
WB
(A-C) Fluorescence analysis, immunoblots and qRT-PCR assays for SHP in macrophages infected by croaker SHP adenovirus.
WB
(B) Immunoblots for phosphorylated AMPK and SHP in macrophages after LPS stimulation alone or with AMPK inhibitor (CC)
WB
(D) Effects of AMPK activator (AICAR) on protein levels of phosphorylated AMPK, phosphorylated ACC and SHP in macrophages.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
