Immunohistochemical
ImmunohistochemicalWestern blot
Host: Rabbit Target Name:ANXA1 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%Western blot
Positive Control: Lane 1: 20ug mouse fibroblast lysates Primary Antibody Dilution : 1:600 Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:2000 Submitted by: AnonymousWestern blot
1.25ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-ANXA1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANX1, LPC1 |
| Immunogen | The immunogen for anti-ANXA1 antibody: synthetic peptide directed towards the N terminal of human ANXA1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePig |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANXA1 |
|---|---|
| Gene Synonyms | ANX1 LPC1 |
| Gene Full Name | Annexin A1 |
| Gene Summary | ANXA1 encodes a protein that belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. |
| Alternative Names | ANX1 LPC1 |
| Molecular Weight(MW) | 38kDa |
| Sequence | 346 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
Host: Rabbit Target Name:ANXA1 Sample Tissue:HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%
Western blot
Positive Control: Lane 1: 20ug mouse fibroblast lysates Primary Antibody Dilution : 1:600 Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:2000 Submitted by: Anonymous
Western blot
1.25ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM107879