Western blot
Positive Control: Lane1: 60ug rat stiatumPrimary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-IRDye800Secondry Antibody Dilution : 1:10,000Submitted by: Ruben van Vugt, The Nijmegen Centre for Molecular Life Sciences (NCMLS)Immunoblot
Lane1: 8 ug total cardiac lysateLane2: 15 ug total cardiac lysateLane3: 30 ug total cardiac lysate Lane4: 50 ug total cardiac lysatePrimary Antibody Dilution: 0.25 ug/mlSecondary Antibody: Secondary Antibody Dilution: Gene Name: GJA1Submitted by: AnonymousWestern blot
Species+tissue/cell type: Total rat cardiac lysate1: 8 ug total cardiac lysate2: 15 ug total cardiac lysate3: 30 ug total cardiac lysate4: 50 ug total cardiac lysatePrimary antibody dilution: 0.25 ug/ml| Product Name | Rabbit anti-GJA1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSS, CX43, GJAL, ODDD, AVSD3, HLHS1, DFNB38 |
| Immunogen | The immunogen for anti-GJA1 antibody: synthetic peptide directed towards the N terminal of human GJA1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Guinea PigHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GJA1 |
|---|---|
| Gene Synonyms | HSS CX43 GJAL ODDD AVSD3 HLHS1 DFNB38 |
| Gene Full Name | Gap junction protein, alpha 1, 43kDa |
| Gene Summary | Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. |
| Alternative Names | HSS CX43 GJAL ODDD AVSD3 HLHS1 DFNB38 |
| Molecular Weight(MW) | 42kDa |
| Sequence | 382 amino acids |
Western blot
Positive Control: Lane1: 60ug rat stiatumPrimary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-IRDye800Secondry Antibody Dilution : 1:10,000Submitted by: Ruben van Vugt, The Nijmegen Centre for Molecular Life Sciences (NCMLS)
Immunoblot
Lane1: 8 ug total cardiac lysateLane2: 15 ug total cardiac lysateLane3: 30 ug total cardiac lysate Lane4: 50 ug total cardiac lysatePrimary Antibody Dilution: 0.25 ug/mlSecondary Antibody: Secondary Antibody Dilution: Gene Name: GJA1Submitted by: Anonymous
Western blot
Species+tissue/cell type: Total rat cardiac lysate1: 8 ug total cardiac lysate2: 15 ug total cardiac lysate3: 30 ug total cardiac lysate4: 50 ug total cardiac lysatePrimary antibody dilution: 0.25 ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
