Western blot
Positive Control: Lane 1: 5ug mouse neural stem cell lysatePrimary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: AnonymousWestern blot
1.25ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-MCM2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN |
| Immunogen | The immunogen for anti-MCM2 antibody: synthetic peptide directed towards the N terminal of human MCM2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MCM2 |
|---|---|
| Gene Synonyms | BM28 CCNL1 CDCL1 cdc19 D3S3194 MITOTIN |
| Gene Full Name | Minichromosome maintenance complex component 2 |
| Gene Summary | The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. |
| Alternative Names | BM28 CCNL1 CDCL1 cdc19 D3S3194 MITOTIN |
| Molecular Weight(MW) | 99kDa |
| Sequence | 904 amino acids |
Western blot
Positive Control: Lane 1: 5ug mouse neural stem cell lysatePrimary Antibody Dilution : 1:1000Secondary Antibody : Anti rabbit - IR-dyeSecondry Antibody Dilution : 1:10,000 Submitted by: Anonymous
Western blot
1.25ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
