Western blot
Host: Rabbit Target Name: Nr5a1 Sample Tissue: Mouse Lung lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-Nr5a1 Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ad4BP, ELP, ELP-3, Ftz-F1, Ftzf1, SF-1, SF1 |
| Immunogen | The immunogen for Anti-Nr5a1 antibody is: synthetic peptide directed towards the C-terminal region of Mouse Nr5a1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Nr5a1 |
|---|---|
| Gene Synonyms | Ad4BP ELP ELP-3 Ftz-F1 Ftzf1 SF-1 SF1 |
| Gene Full Name | nuclear receptor subfamily 5, group A, member 1 |
| Gene Summary | Nr5a1 is a transcriptional activator. It seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. It binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Nr5a1 also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Nr5a1 binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Nr5a1 is activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. |
| Alternative Names | Ad4BP ELP ELP-3 Ftz-F1 Ftzf1 SF-1 SF1 |
| Molecular Weight(MW) | 52kDa |
| Sequence | 462 amino acids |
Western blot
Host: Rabbit Target Name: Nr5a1 Sample Tissue: Mouse Lung lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
