Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Product Name | Rabbit anti-WT1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AWT1, GUD, WAGR, WIT-2, WT33, NPHS4, EWS-WT1 |
| Immunogen | The immunogen for anti-WT1 antibody: synthetic peptide directed towards the N terminal of human WT1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WT1 |
|---|---|
| Gene Synonyms | AWT1 GUD WAGR WIT-2 WT33 NPHS4 EWS-WT1 |
| Gene Full Name | Wilms tumor 1 |
| Gene Summary | WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. |
| Alternative Names | AWT1 GUD WAGR WIT-2 WT33 NPHS4 EWS-WT1 |
| Molecular Weight(MW) | 54kDa |
| Sequence | 497 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
