Western blot
Application: ChIPSample type: mouse liver tissueChromatin Used: 100ug tissueAntibody Used: 10ug Image Submitted by: Joanna DiSpirito, University of PennsylvaniaWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain| Product Name | Rabbit anti-THRB polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBA-BETA, ERBA2, GRTH, MGC126109, MGC126110, NR1A2, THR1, THRB1, THRB2, PRTH, C-ERBA-2, C-ERBA-BETA |
| Immunogen | The immunogen for anti-THRB antibody: synthetic peptide directed towards the N terminal of human THRB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBChIP |
| WB:1:500~1:2000 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | THRB |
|---|---|
| Gene Synonyms | ERBA-BETA ERBA2 GRTH MGC126109 MGC126110 NR1A2 THR1 THRB1 THRB2 PRTH C-ERBA-2 C-ERBA-BETA |
| Gene Full Name | Thyroid hormone receptor, beta |
| Gene Summary | THRB is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Defects in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several transcript variants have been observed for this gene, but the full-length nature of only one has been determined so far. The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. |
| Alternative Names | ERBA-BETA ERBA2 GRTH MGC126109 MGC126110 NR1A2 THR1 THRB1 THRB2 PRTH C-ERBA-2 C-ERBA-BETA |
| Molecular Weight(MW) | 53kDa |
| Sequence | 461 amino acids |
Western blot
Application: ChIPSample type: mouse liver tissueChromatin Used: 100ug tissueAntibody Used: 10ug Image Submitted by: Joanna DiSpirito, University of Pennsylvania
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
