Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Thymus| Product Name | Rabbit anti-E2F3 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F-3 |
| Immunogen | The immunogen for anti-E2F3 antibody: synthetic peptide directed towards the C terminal of human E2F3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F3 |
|---|---|
| Gene Synonyms | E2F-3 |
| Gene Full Name | E2F transcription factor 3 |
| Gene Summary | E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. |
| Alternative Names | E2F-3 |
| Molecular Weight(MW) | 37kDa |
| Sequence | 334 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Thymus| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
