Immunofluorescence
Three cryosections on each slide were stained with a single antibody. The fourth slice was used as a negative control (primary antibody omitted). The tissue was fixed with cold acetone for 10 minutes, rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.Rabbit anti human antibodies and dilutions used:e) ZNF133 (38332), most suitable concentration 1:100Western blot
0.2-1 ug/ml| Product Name | ZNF133 antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-ZNF133 antibody: synthetic peptide directed towards the middle region of human ZNF133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IF |
| Species Reactivity | Human,Pig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZNF133 |
|---|---|
| Gene Synonyms | ZNF150 pHZ-13 pHZ-66 |
| Gene Full Name | Zinc finger protein 133 |
| Gene Summary | ZNF193 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 5 C2H2-type zinc fingers and 1 SCAN box domain. ZNF193 may be involved in transcriptional regulation. |
| Alternative Names | ZNF150 pHZ-13 pHZ-66 |
| Molecular Weight(MW) | 73kDa |
| Sequence | 653 amino acids |
Immunofluorescence
Three cryosections on each slide were stained with a single antibody. The fourth slice was used as a negative control (primary antibody omitted). The tissue was fixed with cold acetone for 10 minutes, rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature.Rabbit anti human antibodies and dilutions used:e) ZNF133 (38332), most suitable concentration 1:100
Western blot
0.2-1 ug/ml| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
