Immunoblot
Lane 1: 40ug whole canine retinaWestern blot
0.2-1 ug/ml| Product Name | XBP1 antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-XBP1 antibody: synthetic peptide directed towards the C terminal of human XBP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Species Reactivity | Bovine,Dog,Human,Mouse,Pig,Rabbit,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | XBP1 |
|---|---|
| Gene Synonyms | TREB5 XBP2 |
| Gene Full Name | X-box binding protein 1 |
| Gene Summary | XBP1 is a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. XBP1 is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. |
| Alternative Names | TREB5 XBP2 |
| Molecular Weight(MW) | 29kDa |
| Sequence | 261 amino acids |
Immunoblot
Lane 1: 40ug whole canine retina
Western blot
0.2-1 ug/ml| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
