Immunohistochemical
Immunohistochemistry of Liver tissue at an antibody concentration of 4-8µg/ml using anti-DGCR8 antibodyWestern blot
Positive Control: Lane 1: Cytoplasmic fraction of mouse back skin lysate Lane 2: Nuclear fraction of mouse back skin lysate Lane 3: Cytoplasmic fraction of mouse back skin lysate Lane 4: Nuclear fraction of mouse back skin lysate Lane 5: Cytoplasmic fraction of mouse back skin lysate Lane 6: Nuclear fraction of mouse back skin lysate Lane 7: Cytoplasmic fraction of DGCR8-ko mouse back skin Lane 8: Nuclear fraction of DGCR8-ko mouse back skin Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Christopher Bennett, University of Colorado-Boulde| Product Name | Rabbit anti-DGCR8 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C22orf12, DGCRK6, Gy1, pasha |
| Immunogen | The immunogen for anti-DGCR8 antibody: synthetic peptide directed towards the N terminal of human DGCR8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DGCR8 |
|---|---|
| Gene Synonyms | C22orf12 DGCRK6 Gy1 pasha |
| Gene Full Name | DiGeorge syndrome critical region gene 8 |
| Gene Summary | DGCR8 contains 2 DRBM (double-stranded RNA-binding) domains and 1 WW domain. It may play a part in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS), a developmental disorder characterized by structural and functional palate anomalies, conotruncal cardiac malformations, immunodeficiency, hypocalcemia, and typical facial anomalies. |
| Alternative Names | C22orf12 DGCRK6 Gy1 pasha |
| Molecular Weight(MW) | 85kDa |
| Sequence | 773 amino acids |
Immunohistochemical
Immunohistochemistry of Liver tissue at an antibody concentration of 4-8µg/ml using anti-DGCR8 antibody
Western blot
Positive Control: Lane 1: Cytoplasmic fraction of mouse back skin lysate Lane 2: Nuclear fraction of mouse back skin lysate Lane 3: Cytoplasmic fraction of mouse back skin lysate Lane 4: Nuclear fraction of mouse back skin lysate Lane 5: Cytoplasmic fraction of mouse back skin lysate Lane 6: Nuclear fraction of mouse back skin lysate Lane 7: Cytoplasmic fraction of DGCR8-ko mouse back skin Lane 8: Nuclear fraction of DGCR8-ko mouse back skin Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Christopher Bennett, University of Colorado-Boulde| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
