Immunoblot
Lane1: 10 ug COS-7 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: ASLSubmitted by: Shawn Elms. Georgia Health Science UniversityWestern blot
5.0ug/mlPositive Control: Human Live| Product Name | Rabbit anti-ASL polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASAL |
| Immunogen | The immunogen for anti-ASL antibody: synthetic peptide directed towards the middle region of human ASL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ASL |
|---|---|
| Gene Synonyms | ASAL |
| Gene Full Name | Argininosuccinate lyase |
| Gene Summary | ASL is a member of the lyase 1 family. The protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in its gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Alternative Names | ASAL |
| Molecular Weight(MW) | 49kDa |
| Sequence | 444 amino acids |
| Entrez Gene | 435 |
|---|---|
| SwissProt ID | P04424 |
| Protein Accession | NP_001020115 |
Immunoblot
Lane1: 10 ug COS-7 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: ASLSubmitted by: Shawn Elms. Georgia Health Science University
Western blot
5.0ug/mlPositive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
