Immunohistochemical
Human A375 cellsPrimary Antibody Dilution :1:100Secondary Antibody :Anti-rabbit-Alexa-546Secondary Antibody Dilution :1:100Color/Signal Descriptions :Red: FGF2 Blue: NucleiGene Name :FGF2Submitted by :Igor Prudovsky, PhD, DSc, Principal Investigator, Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Dr. Scarborough, ME 4074Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-FGF2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BFGF, FGFB, HBGF-2 |
| Immunogen | The immunogen for anti-FGF2 antibody: synthetic peptide directed towards the middle region of human FGF2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FGF2 |
|---|---|
| Gene Synonyms | BFGF FGFB HBGF-2 |
| Gene Full Name | Fibroblast growth factor 2 (basic) |
| Gene Summary | The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | BFGF FGFB HBGF-2 |
| Molecular Weight(MW) | 31kDa |
| Sequence | 288 amino acids |
Immunohistochemical
Human A375 cellsPrimary Antibody Dilution :1:100Secondary Antibody :Anti-rabbit-Alexa-546Secondary Antibody Dilution :1:100Color/Signal Descriptions :Red: FGF2 Blue: NucleiGene Name :FGF2Submitted by :Igor Prudovsky, PhD, DSc, Principal Investigator, Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Dr. Scarborough, ME 4074
Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
