Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: A549 cell lysate| Product Name | Rabbit anti-MGMT polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-MGMT antibody: synthetic peptide directed towards the middle region of human MGMT |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MGMT |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | O-6-methylguanine-DNA methyltransferase |
| Gene Summary | MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA.MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Molecular Weight(MW) | 22kDa |
| Sequence | 207 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: A549 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM110712