Immunohistochemical
Antibody Titration: 2-10ug/mlPositive Control: Human Intestine cell lysateWestern blot
Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate Primary Antibody Dilution : 1:500 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:2000 Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland BaltimoreWestern blot
Sample Type: Equine Cartilage ExplantsPrimary Dilution: 1:800Secondary Antibody: Bio-Rad 170-5046Secondary Dilution::100,000Image Submitted By: Adam WilliamsUniversity of NottinghamWestern blot
1.25ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-MMP1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLG, CLGN |
| Immunogen | The immunogen for anti-MMP1 antibody: synthetic peptide directed towards the N terminal of human MMP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HorseHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MMP1 |
|---|---|
| Gene Synonyms | CLG CLGN |
| Gene Full Name | Matrix metallopeptidase 1 (interstitial collagenase) |
| Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP1 is a secreted enzyme which breaks down the interstitial collagens, types I, II, and III.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| Alternative Names | CLG CLGN |
| Molecular Weight(MW) | 52kDa |
| Sequence | 469 amino acids |
Immunohistochemical
Antibody Titration: 2-10ug/mlPositive Control: Human Intestine cell lysate
Western blot
Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate Primary Antibody Dilution : 1:500 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:2000 Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore
Western blot
Sample Type: Equine Cartilage ExplantsPrimary Dilution: 1:800Secondary Antibody: Bio-Rad 170-5046Secondary Dilution::100,000Image Submitted By: Adam WilliamsUniversity of Nottingham
Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
