Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Pancreas| Product Name | Rabbit anti-Btrc polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta-Trcp1, FWD1, Fbw1a, KIAA4123, Slimb, b-TrCP, beta-TrCP, mKIAA4123, HOS |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanPigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Btrc |
|---|---|
| Gene Synonyms | Beta-Trcp1 FWD1 Fbw1a KIAA4123 Slimb b-TrCP beta-TrCP mKIAA4123 HOS |
| Gene Full Name | Beta-transducin repeat containing protein |
| Gene Summary | Btrc is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. |
| Alternative Names | Beta-Trcp1 FWD1 Fbw1a KIAA4123 Slimb b-TrCP beta-TrCP mKIAA4123 HOS |
| Molecular Weight(MW) | 69kDa |
| Sequence | 605 amino acids |
| Entrez Gene | 12234 |
|---|---|
| SwissProt ID | Q3ULA2 |
| Protein Accession | NP_001032847 |
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Pancreas| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
