Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using HDAC9 antibody - C-terminal region and HCT116 CellsWestern blot
1.25ug/mlPositive Control: A172 cell lysateImmunohistochemical
Immunohistochemical| Product Name | Rabbit anti-HDAC9 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR, HD9, HD7b |
| Immunogen | The immunogen for anti-HDAC9 antibody: synthetic peptide directed towards the C terminal of human HDAC9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMouseRabbitSheep |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HDAC9 |
|---|---|
| Gene Synonyms | DKFZp779K1053 HD7 HDAC HDAC7 HDAC7B HDAC9B HDAC9FL HDRP KIAA0744 MITR HD9 HD7b |
| Gene Full Name | Histone deacetylase 9 |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 may play a role in hematopoiesis.Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. |
| Alternative Names | DKFZp779K1053 HD7 HDAC HDAC7 HDAC7B HDAC9B HDAC9FL HDRP KIAA0744 MITR HD9 HD7b |
| Molecular Weight(MW) | 65kDa |
| Sequence | 590 amino acids |
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using HDAC9 antibody - C-terminal region and HCT116 Cells
Western blot
1.25ug/mlPositive Control: A172 cell lysate
Immunohistochemical
Immunohistochemical| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
