Immunohistochemical
Human HT29 cellsPrimary Antibody Dilution :1:50Secondary Antibody :Donkey anti Rabbit-Alexa Fluor 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: Symplekin Blue: DAPIGene Name :SYMPKSubmitted by :Louise Lagerqvist (PhD), Oncology Department, Institute of Functional Genomics, Montpellier, France.Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Live| Product Name | Rabbit anti-SYMPK polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ27092, SPK, SYM |
| Immunogen | The immunogen for anti-SYMPK antibody: synthetic peptide directed towards the N terminal of human SYMPK |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYMPK |
|---|---|
| Gene Synonyms | FLJ27092 SPK SYM |
| Gene Full Name | Symplekin |
| Gene Summary | SYMPK is a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | FLJ27092 SPK SYM |
| Molecular Weight(MW) | 141kDa |
| Sequence | 1274 amino acids |
Immunohistochemical
Human HT29 cellsPrimary Antibody Dilution :1:50Secondary Antibody :Donkey anti Rabbit-Alexa Fluor 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :Green: Symplekin Blue: DAPIGene Name :SYMPKSubmitted by :Louise Lagerqvist (PhD), Oncology Department, Institute of Functional Genomics, Montpellier, France.
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
