Immunoblot
Lane 1: 40ug canine retina lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-rabbit-HRPSecondary Antibody Dilution: 1:5000Gene Name: STUB1Submitted by: Dr. Stefania Marsili and Dr. William Beltran, University of PennsylvaniaImmunoblot
1:1ug insoluble STUB1 protein, 2:1ug soluble STUB1 protein, 3:1ug EPM2A protein, 4:1ug insoluble PPP1R3C protein, 5:1ug soluble PPP1R3C proteinPrimary Antibody Dilution: 1:2500Secondary Antibody: Anti-rabbit-APSecondary Antibody Dilution: 1:20,000Gene Name: STUB1Submitted by: Pedro Castanheira, BiocantWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Transfected 293T| Product Name | Rabbit anti-STUB1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHIP, HSPABP2, NY-CO-7, SDCCAG7, UBOX1 |
| Immunogen | The immunogen for anti-STUB1 antibody: synthetic peptide directed towards the C terminal of human STUB1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STUB1 |
|---|---|
| Gene Synonyms | CHIP HSPABP2 NY-CO-7 SDCCAG7 UBOX1 |
| Gene Full Name | STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase |
| Gene Summary | STUB1 modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It has E3 ubiquitin-protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. STUB1 mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. |
| Alternative Names | CHIP HSPABP2 NY-CO-7 SDCCAG7 UBOX1 |
| Molecular Weight(MW) | 35kDa |
| Sequence | 303 amino acids |
Immunoblot
Lane 1: 40ug canine retina lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-rabbit-HRPSecondary Antibody Dilution: 1:5000Gene Name: STUB1Submitted by: Dr. Stefania Marsili and Dr. William Beltran, University of Pennsylvania
Immunoblot
1:1ug insoluble STUB1 protein, 2:1ug soluble STUB1 protein, 3:1ug EPM2A protein, 4:1ug insoluble PPP1R3C protein, 5:1ug soluble PPP1R3C proteinPrimary Antibody Dilution: 1:2500Secondary Antibody: Anti-rabbit-APSecondary Antibody Dilution: 1:20,000Gene Name: STUB1Submitted by: Pedro Castanheira, Biocant
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Transfected 293T| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
