Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysate| Product Name | Rabbit anti-RAD18 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNF73 |
| Immunogen | The immunogen for anti-RAD18 antibody: synthetic peptide directed towards the middle region of human RAD18 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAD18 |
|---|---|
| Gene Synonyms | RNF73 |
| Gene Full Name | RAD18 homolog (S. cerevisiae) |
| Gene Summary | RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. |
| Alternative Names | RNF73 |
| Molecular Weight(MW) | 56kDa |
| Sequence | 495 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
