Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using Hdac4 Antibody - C-terminal region and HCT116 CellsImmunoblot
Lane 1: 40ug mouse brain, synaptosome lysate Lane 2: 40ug mouse brain, membrane fraction Lane 3: 40ug mouse brain, cytoplasm fraction Lane 4: 40ug mouse brain, nuclear fraction Lane 5: 40ug mouse brain, post synaptic density fraction Lane 6: 40ug mouse brain, synaptosome lysate Lane 7: 40ug mouse brain, membrane fraction Lane 8: 40ug mouse brain, cytoplasm fraction Lane 9: 40ug mouse brain, nuclear fraction Primary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: Hdac4Submitted by: Wen-Cheng Xiong, Georgia Health Sciences UniversityWestern blot
Titration: 1.0 ug/mlPositive Control: Mouse Live| Product Name | Rabbit anti-Hdac4 Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4932408F19Rik, AI047285 |
| Immunogen | The immunogen for Anti-Hdac4 antibody is: synthetic peptide directed towards the C-terminal region of Mouse Hdac4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Hdac4 |
|---|---|
| Gene Synonyms | 4932408F19Rik AI047285 |
| Gene Summary | Hdac4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. |
| Alternative Names | 4932408F19Rik AI047285 |
| Molecular Weight(MW) | 118kDa |
| Sequence | 1076 amino acids |
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using Hdac4 Antibody - C-terminal region and HCT116 Cells
Immunoblot
Lane 1: 40ug mouse brain, synaptosome lysate Lane 2: 40ug mouse brain, membrane fraction Lane 3: 40ug mouse brain, cytoplasm fraction Lane 4: 40ug mouse brain, nuclear fraction Lane 5: 40ug mouse brain, post synaptic density fraction Lane 6: 40ug mouse brain, synaptosome lysate Lane 7: 40ug mouse brain, membrane fraction Lane 8: 40ug mouse brain, cytoplasm fraction Lane 9: 40ug mouse brain, nuclear fraction Primary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: Hdac4Submitted by: Wen-Cheng Xiong, Georgia Health Sciences University
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
