Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: NCI-H226 cell lysate| Product Name | Rabbit anti-CDC27 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC3, CDC27Hs, D0S1430E, D17S978E, HNUC, NUC2, ANAPC3 |
| Immunogen | The immunogen for anti-CDC27 antibody: synthetic peptide directed towards the middle region of human CDC27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC27 |
|---|---|
| Gene Synonyms | APC3 CDC27Hs D0S1430E D17S978E HNUC NUC2 ANAPC3 |
| Gene Full Name | Cell division cycle 27 homolog (S. cerevisiae) |
| Gene Summary | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | APC3 CDC27Hs D0S1430E D17S978E HNUC NUC2 ANAPC3 |
| Molecular Weight(MW) | 92kDa |
| Sequence | 824 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: NCI-H226 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
